The Source Wholesale Colour Changing Clam Light

Product Information

Alcazar, S. N. (1986). Observations on predators of giant clams (Bivalvia: Family Tridacnidae). Silliman J. 33, 54–57. You’ll want this light to be in front of you (i.e., the photographer), so that your camera is able to sit just below it for a straight or raised angle. Mohan, K., Purnapatra, S. B. & Mondal, P. P. Three dimensional fluorescence imaging using multiple light-sheet microscopy. PLoS ONE 9, e96551 (2014). While you only need two lights to create the clamshell pattern, you’re always free to add additional lights to the setup. Stand behind the lights and shoot through the gap. Note: If there isn’t much of a gap to work with, raise and/or lower both of your lights until you have enough room to shoot in the middle. To be safe, you may want to take another meter reading.

Now let’s take a look at some clamshell examples. You can use them as inspiration, though don’t limit yourself – these are just a handful of the many clamshell setups you can create! Banaszak, A. T., LaJeunesse, T. C., and Trench, R. K. (2000). The synthesis of mycosporine-like amino acids (MAAs) by cultured, symbiotic dinoflagellates. J. Exp. Mar. Biol. Ecol. 249, 219–233. doi: 10.1016/s0022-0981(00)00192-1 Also note that, while clamshell lighting does offer a lot of flexibility in terms of positioning shadows, it’s generally not very slimming; most of the shadows under the subject’s chin are pushed away by the bottom clamshell light, so a better alternative (when the goal is to slim down the subject’s face) is to go with butterfly lighting. Clamshell Lighting: A Step-By-Step GuideIf you’re not a fan of the bottom catchlight, don’t fret! It can always be cloned out during post-processing. Clamshell lighting examples Núñez-Pons, L., Avila, C., Romano, G., Verde, C., and Giordano, D. (2018). UV-protective compounds in marine organisms from the southern ocean. Mar. Drugs 16:336. doi: 10.3390/md16090336 Clamshell lighting requires two light sources, and these can be strobes or continuous, modified or unmodified.

Using a reflector is an easy way to create clamshell lighting with minimal gear. However, with the subject holding the reflector in place, you may be more limited in your poses and composition. If the reflector is too limiting, you can use a second studio light as the fill instead. interior of a modern photo studio Use a Second Studio Light Klumpp, D., and Griffiths, C. (1994). Contributions of phototrophic and heterotrophic nutrition to the metabolic and growth requirements of four species of giant clam ( Tridacnidae). Mar. Ecol. Prog. Ser. 115, 103–115. doi: 10.3354/meps115103 Kawanishi, S., Hiraku, Y., and Oikawa, S. (2001). Mechanism of guanine-specific DNA damage by oxidative stress and its role in carcinogenesis and aging. Mutat. Res. Rev. Mutat. Res. 488, 65–76. doi: 10.1016/s1383-5742(00)00059-4 At this point, you’ll have a setup that’ll produce a lot of angular, intense shadows, but this isn’t what clamshell lighting is about. That’s why you need to add a reflector under your subject’s chin, or a second light, one that will push light beneath your subject and create a stunning result. You should generally start with this light at a 45-degree angle, pointing up, so that it mirrors the key light, though you’ll then be able to make adjustments depending on your subject and the shot you’ve envisioned.Andréfouët, S., Gilbert, A., Yan, L., Remoissenet, G., Payri, C., and Chancerelle, Y. (2005). The remarkable population size of the endangered clam Tridacna maxima assessed in Fangatau Atoll (Eastern Tuamotu. French Polynesia) using in situ and remote sensing data. ICES J. Mar. Sci. 62, 1037–1048. doi: 10.1016/j.icesjms.2005.04.006 Prevedel, R. et al. Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy. Nat. Methods 11, 727–730 (2014). We thank Prof. Zhiping Lai for his help with experimental materials and the “Imaging and Characterization Core Lab” of KAUST for support with the SEM and TEM imaging. Supplementary Material Light position matters in clamshell lighting, and even an adjustment of a few inches can dramatically change the final image.

Goessling, J. W., Wardley, W. P., and Lopez-Garcia, M. (2019). Natural slab photonic crystals in centric diatoms. bioRxiv [Preprint]. doi: 10.1101/838185 Funding: DK- Brown Foundation, University of Arizona Postdoctoral Fellowship DT- University of Arizona RII The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Keller, P. J., Schmidt, A. D., Wittbrodt, J. & Stelzer, E. H. K. Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy. Science 322, 1065–1069 (2008). Carreja, B., Fernández, M., and Agustí, S. (2016). Joint additive effects of temperature and UVB radiation on zoeae of the crab Taliepus dentatus. Mar. Ecol. Prog. Ser. 550, 135–145. doi: 10.3354/meps11715Note that both the key light and the second ( fill) light should now both be in front of you, the photographer–and your aim is generally to shoot the model from head-on, with a lens that pokes out from between the two light sources. Of course, you can ask the model to turn their head and strike different poses, but be careful to maintain the same shadow presence that you see from very straight clamshell lighting. You can use any type of modifier that softens the light. An umbrella or beauty dish will also work well.

We have demonstrated a new volumetric imaging platform, CLAM, that exploits a complete parallelized 3D multiplexed LSFM strategy. In contrast to the existing LSFM modalities, the defining feature of CLAM is its generation of a dense light-sheet array (>30), which is reconfigurable in both temporal coherency and spatial density. Furthermore, the 3D imaging throughput and efficiency can be maximized (spatial duty cycle of 100%) by multiplexed light-sheet coding (i.e., OFDM). The combination of these two features enables the simultaneous capture of all-optically sectioned image planes in a continuous volume at >10 vol/s, obviating the need for bulky objective scanning or beam scanning/dithering. CLAM does not have a fundamental limitation in scaling to a higher volume rate as sCMOS technology continually advances (e.g., >10,000 fps in a state-of-the-art sCMOS camera) 34. More importantly, CLAM simultaneously reads out all of the voxels in three dimensions and thus allows a longer integration (exposure) time and potentially better SNR, especially for sparse samples. CLAM thus reduces the illumination power and outperforms other LSFM modalities in reducing the photobleaching/phototoxicity. CLAM only replaces the beam scanning module by the mirror pair (for parallelized illumination) and the spinning reticle (for parallelized encoding). Hence, CLAM can readily be compatible with any existing LSFM modalities with minimal hardware modification or dedicated synchronization. In this regard, CLAM could easily be further advanced to tailor specific applications. For instance, parallelized discrete light-sheet array illumination also provides another degree of freedom to arbitrarily select subsets of light sheets. This could be of particular interest in sparse sampling of neuronal activity recording in brain imaging applications 1, 2. Simultaneous multi-view CLAM can also be implemented by illumination with the light-sheet array from multiple directions—an effective strategy that has been proven to improve the image quality in the presence of light scattering and resolution isotropy 41. CLAM should also be compatible with the available wavefront coding/shaping techniques used for increasing the FOV in both the axial and lateral dimensions 4, 42. Notably, CLAM, when combined with additional spatial light modulation and/or a beam scanning module, can be adopted to create a more sophisticated structured illumination, such as a lattice light sheet 12, 43, 44. CLAM can also be combined with an adaptive optics module to overcome image distortion and aberration in deep tissue in vivo 11, 45, which could impact applications in neurobiology. Cloney, R. A., and Brocco, S. L. (1983). Chromatophore organs, reflector cells, iridocytes and leucophores in cephalopods. Am. Zool. 23, 581–592. doi: 10.1093/icb/23.3.581Once you’ve created the “clamshell” setup, with your lights nicely positioned in the shape of a clam, you’ll want to fine tune the strength of the lights.